红景天预处理对大鼠肝脏缺血再灌注损伤细胞凋亡的影响

目的：探讨胃饲红景天预处理对大鼠肝脏缺血再灌注损伤后细胞凋亡的影响及其对肝功能的保护作用。方法：48只健康成年雄性SD大鼠随机分为生理盐水对照组和红景天预处理组,两组分别予与等量生理盐水、红景天水煎剂（5g·kg^-1·d^-1生药材）灌胃5d,末次灌胃2h后经第一肝门阻断入肝血流30min而后松开无创动脉夹,恢复人肝血流,分别于缺血30min及再灌注后1h、6h及24h各时相点活杀动物取材,检测血清丙氨酸氨基转移酶（ALT）、肝组织丙二醛（MDA）,并用TUNEL染色及图像分析法检测肝细胞凋亡率的变化。结果：与生理盐水对照组比较,红景天预处理组ALT、MDA、肝细胞凋亡率在上述多个时相点显著降低（P〈0．05）。结论：肝脏缺血再灌注前给予红景天水煎剂预处理可以显著降低肝细胞凋亡率,减少MDA含量,并能有效地保护肝脏功能。     

红景天治疗大鼠心肌肥厚及对转化生长因子β_1表达影响

目的:观察红景天对大鼠压力超负荷型心肌肥厚的作用,探讨其对转化生长因子(TGF-β1)表达的影响。方法:通过不完全结扎大鼠腹主动脉法构建大鼠心肌肥厚模型,将SD大鼠随机分为4组每组10只。给药组动物术前1周始灌胃给予相应剂量红景天(150mg.kg-1.d-1或300mg.kg-1.d-1)直至术后4周。给药第5周末,测定各组大鼠血压和心脏质量指数(HMI);观察心肌组织结构;检测左室心肌组织中TGF-β1 mRNA表达。结果:红景天给药组动物的血压、HMI、光镜下的心肌细胞肥大程度和左室心肌组织中TGF-β1 mRNA表达较单纯手术组均有显著下降(P<0.01或P<0.05)。结论:红景天可有效的缓解压力超负荷诱导的大鼠心肌肥厚,通过降低左室心肌TGF-β1表达,影响TGF-β信号转导产生作用。     

复方甘草酸单铵治疗玫瑰糠疹疗效观察

目的探讨复方甘草酸单铵治疗玫瑰糠疹的疗效及其安全性。方法70例玫瑰糠疹患者分为2组,治疗组40例,给予复方甘草酸单铵注射液40ml,ivgtt,qd,;对照组30例,给予左西替力嗪5mg,qd,两组均外用炉甘石洗剂,每天2次;10d为一疗程。结果治疗组痊愈率及总有效率分别为72．5％和90．00％,对照组分别为53．33％和66．67％,两组比较均有显著性差异（P〈0．01）,两组均未见明显不良反应。结论复方甘草酸单铵治疗玫瑰糠疹疗效确切,疗程短,不良反应少,值得临床应用。     

玫瑰糠疹发病机制的研究进展

玫瑰糠疹是一种常见的急性炎症性皮肤病,典型皮损为覆有领圈状糠状鳞屑的玫瑰色斑疹,病程有自限性,发病机制至今尚未阐明。本文综合近年国内外研究资料,就淋巴细胞、朗汉斯细胞及一些细胞因子在玫瑰糠疹发病中的作用进行了综述,归纳了其可能的发病机制,即机体被细菌或病毒感染后,由朗汉斯细胞启动,活化淋巴细胞,产生细胞因子,导致机体发生迟发型变态反应。     

Antifertility Activity of Stems of Plumbago rosea in Female Albino Rats

In the present study, Plumbago rosea Linn. (Plumbaginaceae), one of the folk medicinal plants commonly used as an antifertility agent, was evaluated for its antifertility effect. Five successive solvent extracts, petroleum ether, chloroform, acetone, ethanol, and water extracts, of the stems of P. rosea were studied on estrous cycle at two dose levels, 200 and 400 mg/kg respectively. Of these, the acetone extract was found to be most effective in interrupting the normal estrous cycle of rats (p < 0.05) (p < 0.01). The rats exhibited prolonged diestrous stage of the estrous cycle with consequent temporary inhibition of ovulation. The antiovulatory activity was reversible on withdrawal of the extract. The effective acetone extract was further studied on estrogenic functionality in rats. The acetone extract showed significant estrogenic and antiestrogenic activity (p < 0.05) (p < 0.001). Histological studies of the uteri further confirmed the estrogenic activity of acetone extract. The results indicated the antifertility activity of stems of Plumbago rosea in female Wistar rats.     

Rhodiola rosea extract protects human cortical neurons against glutamate and hydrogen peroxide-induced cell death through reduction in the accumulation of intracellular calcium

The aim of this study was to investigate the neuroprotective effects of a titolated extract from Rhodiola rosea L. (RrE) and of salidroside (Sa), one of the major biologically active compounds extracted from this medicinal plant, against oxidative stressor hydrogen peroxide (H₂O₂) and glutamate (GLU)‐induced cell apoptosis in a human cortical cell line (HCN 1‐A) maintained in culture. The results obtained indicate that exposure of differentiated HCN 1‐A neurons to GLU or H₂O₂ resulted in concentration‐dependent cell death. A 24 h pre‐treatment with RrE significantly increased cell survival and significantly prevented the plasma membrane damage and the morphological disruption caused by GLU or H₂O₂, indicating that neurons treated with RrE were protected from the neurotoxicity induced by the oxidative stressor used. In addition, RrE significantly reduced H₂O₂ or GLU‐induced elevation of intracellular free Ca²⁺ concentration. The results obtained have also shown that Sa caused similar effects in all experimental models used; however, the potency of the action was lower than that of the extract containing corresponding quantities of Sa. These findings indicate that RrE has a neuroprotective effect in cortical neurons and suggest that the antioxidant activity of the RrE, due to the structural features of the synergic active principles they contain, may be responsible for its ability to stabilize cellular Ca²⁺ homeostasis. Copyright © 2011 John Wiley & Sons, Ltd.     

窄谱中波紫外线照射联合复方甘草酸苷治疗玫瑰糠疹疗效分析

目的观察窄谱中波紫外线照射联合复方甘草酸苷治疗玫瑰糠疹的疗效。方法对照组采用窄谱中波紫外线照射(2天一次)治疗,治疗组采用窄谱中波紫外线照射联合复方甘草酸苷(2片3次/d口服)治疗,两组均20天为1个疗程。结果治疗组有效率为95.83%,对照组为66.67%,治疗组疗效优于对照组,两组间差异有显著性(P<0.05);治疗组不良反应发生率为16.67%,对照组为20.83%,治疗组不良反应略少于对照组,但两组间差异无显著性(P>0.05)。结论窄谱中波紫外线照射联合复方甘草酸苷治疗玫瑰糠疹疗效好,安全可靠。     

黄芩苷与红景天胶囊对急性高原病预防作用比较

 目的 探讨黄芩苷胶囊对急性高原病(acute mountain sickness, AMS)的预防作用。方法 采用随机对照的研究方法, 80名急进高原健康男性青年随机分为3组, 黄芩苷组(n=32)、红景天组(n=24)和安慰剂组(n=24)。3组在进入高原前2 d、进入高原后连续3 d分别服用黄芩苷胶囊(0.5 g, 2次/d)、红景天胶囊(0.76 g, 2次/d)和安慰剂(2粒, 2次/d)。检测急进高原前(海拔397 m)和急进高原后(3658 m)受试者氧饱和度、心率、收缩压、舒张压, 彩色多普勒超声测量肺动脉收缩压(pulmonary artery systolic pressure, PASP)和平均肺动脉压(mean pulmonary arterial pressure, MPAP), 统计急进高原后各组的AMS发病率。结果 急进高原后安慰剂组、黄芩苷组和红景天组的AMS发病率分别为58.3%(14/24)、25.0%(8/32)和29.2%(7/24), 与安慰剂组比较, 黄芩苷组及红景天组AMS发病率明显降低, 差异有统计学意义(P<0.05)。与急进高原前比较, 各组受试者急进高原后心率、血压及肺动脉压均升高, 氧饱和度降低(P<0.05);与安慰剂组比较, 黄芩苷组血压及心率明显降低(P<0.05), 红景天组急进高原后氧饱和度升高、肺动脉压降低(P<0.05)。结论 黄芩苷组可能通过降低血压及心率预防AMS的发生, 红景天胶囊可能通过升高氧饱和度, 降低PASP、MPAP预防AMS的发生。     

白细胞介素22在玫瑰糠疹患者外周血单一核细胞及血清中的表达

【摘要】 目的 探讨白细胞介素（IL）-22在玫瑰糠疹发病机制中的作用。 方法 荧光实时定量反转录（RT）-PCR检测玫瑰糠疹患者急性期、恢复期及健康对照组PBMC中IL-22mRNA的表达水平。采用酶联免疫吸附试验检测血清中IL-22的水平。 结果 玫瑰糠疹患者急性期、恢复期及健康对照组PBMC中IL-22 mRNA的相对表达量分别为4.15 ± 1.36、2.89 ± 0.91和1.91 ± 0.60,急性期明显高于恢复期（t = 3.61,P ＜ 0.05）,恢复期高于健康对照组（t = 3.84,P ＜ 0.05）。玫瑰糠疹患者急性期、恢复期及健康对照组血清中IL-22水平分别为（36.87 ± 10.63）、（21.44 ± 7.33）和（13.22 ± 4.26） ng/L,急性期明显高于恢复期（t = 5.01,P ＜ 0.05）,恢复期高于健康对照组（t = 4.49,P ＜ 0.05）。血清IL-22水平与玫瑰糠疹病程呈正相关（r = 0.883,P ＜ 0.05）。结论 IL-22在玫瑰糠疹患者外周血及血清中表达明显升高,可能在玫瑰糠疹的发病中起一定作用。     

Viral exanthems in childhood. Part 3: Parainfectious exanthems and those associated with virus‐drug interactions

Viruses cause not only direct infectious exanthems, but also parainfectious exanthems, which provoke skin alterations via interactions with the immune system. These distinct exanthems, for instance Gianotti‐Crosti syndrome and pityriasis lichenoides group, do not reflect a specific pathogen but can occur in the course of many viral infections. In addition, some exanthems result from the interaction between viruses and drugs.